Capillary Electrophoresis of Carbohydrates (Methods in by Pierre Thibault, Susumu Honda

By Pierre Thibault, Susumu Honda

A set of state of the art options for utilizing capillary electrophoresis (CE) to investigate complicated carbohydrates. those conveniently reproducible protocols offer equipment for pattern coaching, research of mono- and oligosaccharides, glycoproteins, and glycoconjugates. an invaluable appendix describes the buildings of the main often encountered carbohydrate residues and olgosaccharides from mammalian and bacterial origins. every one protocol comprises distinct details on reagents, gear, notes, reviews, and pointers on systems.

Show description

Read Online or Download Capillary Electrophoresis of Carbohydrates (Methods in Molecular Biology Vol 213) PDF

Similar diagnostics & labs books

Onychomycosis : the current approach to diagnosis and therapy

Content material: Epidemiology 2. Anatomy of the conventional Nail three. medical styles Correlated with major Routes of access four. scientific Diferential analysis five. Mycological exam 6. ambitions for the remedy of Onychomycosis 7. overview of present Antifungal remedy eight. Preventative Measures nine. Index

Histotechnik: Praxislehrbuch für die Biomedizinische Analytik

Dieses einmalige Buch zur Histotechnik stellt die unterschiedlichen Techniken, die in einem histologischen exertions angewandt werden, umfassend vor. Diese zweite überarbeitete und aktualisierte Ausgabe wurde um Techniken ergänzt, die in den letzten Jahren entwickelt wurden. So finden sich neben Basisthemen wie Fixierung, Gewebe-Processing und Färbung, die Grundlagen der Immunhistochemie und in-situ-Hybridisierung sowie aktuelle Kapitel zur Molekularpathologie.

Holographic Sensors

This thesis provides a theoretical and experimental strategy for the speedy fabrication, optimization and trying out of holographic sensors for the quantification of pH, natural solvents, steel cations, and glucose in strategies. constructing non-invasive and reusable diagnostics sensors that may be simply synthetic will help the tracking of high-risk contributors in any scientific or point-of-care surroundings.

Extra info for Capillary Electrophoresis of Carbohydrates (Methods in Molecular Biology Vol 213)

Example text

Enrichment of electron density on the nitrogen atom of the amine reagent by introducing an electron-donating group such as a methyl group enhances the reactivity of the reductive amination and gives higher yields of N-methylglycamines (MGs) under much milder conditions (Fig. 8). The reaction can be carried out in an aqueous medium, and the resulting glycamines can be labeled by 7-nitro-2,1,3-benzoxadiazole (NBD) following 52 Suzuki et al. the condensation with NBD 4-fluoride (NBD-F). The presence of the methyl group on the nitrogen atom is again beneficial for accelerating this condensation, giving higher yields of NBD-tagged MGs in a short time.

Discard the upper layer and add again the same volume of ethyl acetate. Repeat this procedure three times in total, and analyze the final aqueous solution by CE. 3. CE: Use an apparatus equipped with a sample introduction device, a high-voltage supply, an argon laser capable of supplying approx 3 mW power (see Note 26), a fluorescence detector, and a data processor. Uncoated fused silica capillaries are commercially available, for example, from Polymicro Technologies, Phoenix, AZ (see Note 27).

Store remaining glycoprotein at 4°C for future use. 7. 4% SDS. 8. Incubate for 3 min at 100°C. 9. Cool and add 50 µL of CHAPS detergent buffer. 10. 2 U (2 µL) of PNGase F. 11. Incubate for 24 h at 37°C (add 5 µL of toluene to prevent bacterial growth). 12. Remove 5 µL and analyze the reaction mixture by SDS-PAGE (see Note 11). 13. If sample is completely deglycosylated proceed with step 14 otherwise continue with incubation. 14. Filter samples through protein binding membrane or perform gel filtration (see Note 12).

Download PDF sample

Rated 4.48 of 5 – based on 24 votes

Related posts