Functional Genomics: Methods and Protocols by Michael J. Brownstein, Arkady Khodursky

By Michael J. Brownstein, Arkady Khodursky

Nationwide Institutes of wellbeing and fitness, Rockville, MD. textual content describes step by step tools for microarray-based reports and offers suggestions in optimum information research and informatics. experiences each one key technique and analytical suggestions utilized in useful genomics. For researchers.

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Add 95 mL of phenol directly to the saturation buffer and mix well. Hydroxyquinoline may be added if desired. Aliquot and freeze at –20°C for long-term storage. This preparation of saturated phenol will have only one phase. 2. Chloroform (cat. no. C2432 or cat. no. C0549; Sigma). 3. Isopropanol (cat. no. I9516; Sigma). 4. Liquid nitrogen or dry ice. 5. , Polytron or equivalent). For <200 mg of tissue, use a 6-mm probe; for >200 mg of tissue, use a 10-mm probe. 6. [α-33P]dATP (10 µCi/µL; >2500 Ci/mmol) (cat.

1, 57–59. , and Sakaki, Y. (1995) High-density cDNA filter analysis: A novel approach for large-scale quantitative analysis of gene expression. Gene 166, 207–213. 30 Jokhadze et al. Plastic Microarrays 31 3 Plastic Microarrays A Novel Array Support Combining the Benefits of Macro- and Microarrays Alexander Munishkin, Konrad Faulstich, Vissarion Aivazachvili, Claire Granger, and Alex Chenchik 1. Introduction Until recently, gene arrays could only be printed on two types of supports: nylon membranes or glass slides.

Affixing a small piece of adhesive tape to the corner of the protective sheet as a “handle” may make it easier to peel the sheet back from the surface of the film. 2. ). 52 Munishkin et al. 3. , pancreas, liver, spleen), we recommend that you perform a third or fourth round of phenolϺchloroform extraction. 4. Impurities in RNA samples can inhibit RT. In this case, you may need to perform additional steps to purify the total RNA starting material. 5 vol of ethanol. This will help ensure the removal of any protein and other impurities that may not have been removed effectively during initial RNA purification.

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