Handbook of Proteomic Methods by P. Michael Conn

By P. Michael Conn

A compendium thirty-four robust innovations for picking and interpreting the range of proteins expressed in cells. Thee comfortably reproducible proteomic equipment variety from common to express ideas, and contain equipment for facts research, posttranslational amendment, and its variations and isoforms. extra equipment show the applying of proteomics to the invention of serological tumor markers, to deciding on the determinants of sensitivity to antitumor medications, and to really expert fields, corresponding to endocrinology, plant biology, nephrology, and urology.

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Ong, S. , et al. (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 1, 376–386. 28. Krishna, R. G. and Wold, F. (1993) Post-translational modification of proteins. Adv. Enzymol. Relat. Areas Mol. Biol. 67, 265–298. 29. , Ong, S. , Jensen, O. , and Pandey, A. (2002) Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 20, 261–268. 30. Maguire, P.

Sci. USA 93, 12142–12149. 5. Washburn, M. P. and Yates, J. R. III. (2000) Analysis of the microbial proteome. Curr. Opin. Microbiol. 3, 292–297. 6. Pandey, A. and Mann, M. (2000) Proteomics to study genes and genomes. Nature 405, 837–846. 7. Lopez, M. F. (2000) Better approaches to finding the needle in a haystack: optimizing proteome analysis through automation. Electrophoresis 21, 1082–1093. 8. , Lacobini, E. , and Grandi, G. (2001) Combined automated PCR cloning, in vitro transcription/translation and twodimensional electrophoresis for bacterial proteome analysis.

Biotechnol. 13, 321. 27. Ong, S. , et al. (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 1, 376–386. 28. Krishna, R. G. and Wold, F. (1993) Post-translational modification of proteins. Adv. Enzymol. Relat. Areas Mol. Biol. 67, 265–298. 29. , Ong, S. , Jensen, O. , and Pandey, A. (2002) Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol.

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