In Vivo Fluorescence Imaging: Methods and Protocols by Mingfeng Bai

By Mingfeng Bai

This targeted quantity encompasses a wealthy number of functions utilizing quite a few instrumentations, probes, ailment types, and ambitions with a view to account for the multidisciplinary nature of using in vivo fluorescence think. The booklet additionally contains chapters at the rising fields of telephone monitoring, image-guided remedy, and fluorescence imaging within the moment NIR window, in addition to protocols for assessment equipment sooner than and after in vivo imaging. Written for the hugely winning Methods in Molecular Biology sequence, chapters contain short introductions to their respective subject matters, lists of the required fabrics and reagents, step by step effortlessly reproducible laboratory protocols, and pointers on troubleshooting and warding off identified pitfalls. 

Authoritative and functional, In Vivo Fluorescence Imaging: equipment and Protocols serves as a worthwhile reference for researchers from a variety of fields who desire to develop into extra accustomed to in vivo fluorescence imaging techniques.

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5 for sample reaction. 20 Xiaoxi Ling Fig. 4 Synthesis of protein kinase Cδ targeting coumarin based fluorophore [4]: (a) Thionyl chloride, THF, cat. DMF; (b) NEt3, THF. Reproduced with permission from Ref. 4. Copyright (2011) American Chemical Society Fig. 5 Synthesis of fluorescent inhibitor of cycloxygenase-2 [5]: (a) NEt3, DCM. Reproduced from Ref. 6 Michael Addition with Maleimide and Thiol 1. 0 mL) under inert atmosphere. 2. After completion of the reaction, remove the solvent and purify the product using the appropriate method.

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Set the flow rate to 4 mL/min, with the mobile phase starting from 95 % solvent A and 5 % solvent B (0–3 min) to 35 % solvent A and 65 % solvent B at 33 min. 12. Collect and lyophilize the product (see Note 8). 2 Probe Synthesis 1. 5 mL centrifuge tube (see Note 9). 2. Keep the tube in dark and shake for 2 h (see Note 10). 3. 5-labeled peptide with RP-HPLC. Set the flow rate to 4 mL/min, with the mobile phase starting from 95 % solvent A and 5 % solvent B (0–3 min) to 35 % solvent A and 65 % solvent B at 33 min.

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