By Subhash C. Basu, Manju Basu
A panel of expert researchers describes in step by step element their most sensible laboratory suggestions for elucidating liposome biology. With those without problems reproducible equipment, investigators can light up such serious questions because the attachment of liposmes to cellphone surfaces, the permeation of liposomes in the course of the plasma membranes, and the steadiness of liposomes in mobile and nuclear matrices. every one distinct protocol is gifted by way of an investigator who's in detail acquainted with its difficulties and functions, and features a precise record of fabrics for use, troubleshooting information instantly from the benchtop, and notes on pitfalls to prevent.
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Extra resources for Liposome Methods and Protocols
For our peptide model system, leakage was found to be negligible using either assay, implying that the membrane-anchored WAE peptide induces genuine and, in terms of membrane integrity, a carefully controlled fusion process. 4. Insight into the Structure–Function Relationship of the Membrane-Anchored Peptide The membrane-anchored WAE peptide model system we developed offers the unique opportunity to study the molecular relationship between structural and functional features at the level of a fusion peptide.
J. Biochem. 193, 409–420. 22. Novick, S. L. and Hoekstra, D. (1988) Membrane penetration of Sendai virus glycoproteins during the early stages of fusion with liposomes as determined by hydrophobic photoafﬁnity labeling. Proc. Natl. Acad. Sci. USA 85, 7433–7437. Liposomes in Protein–Lipid Studies 49 4 Liposomes Applications in Protein–Lipid Interaction Studies Sujoy Ghosh and Robert Bell 1. Introduction Liposomes are synthetic mimics of cellular membranes and represent an experimental system widely used for more than 30 yr in the ﬁeld of biochemical research involving lipids.
5. ) in cultured cells (17,18). Cationic liposomes often offer an experimental advantage in delivery efﬁciency over other existing methods such as calcium phosphate transfection, scrape loading, etc. These liposomes are now commercially available from several vendors. The focus of the present chapter is on the application of liposomes toward understanding protein–lipid interactions. ) Our experience with protein–lipid interaction is built on studies performed with protein kinase C and Raf-1 kinase.