Multidimensional Liquid Chromatography Theory and by Steven A. Cohen, Mark R. Schure

By Steven A. Cohen, Mark R. Schure

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9. Reprinted with permission from Murphy et al. (1998) by courtesy of the American Chemical Society. ORTHOGONALITY 19 This approach is reviewed in full detail for 1D chromatography by Schoenmakers (1986) and extended by Schure (1999) for dimensions of two and larger. The peak amplitudes f and g are determined either through programmed search or through inspection in a spreadsheet program. Both quantities are corrected for a finite baseline amplitude. It is interesting that this approach works for 1D, 2D, 3D and so on.

ORTHOGONALITY 19 This approach is reviewed in full detail for 1D chromatography by Schoenmakers (1986) and extended by Schure (1999) for dimensions of two and larger. The peak amplitudes f and g are determined either through programmed search or through inspection in a spreadsheet program. Both quantities are corrected for a finite baseline amplitude. It is interesting that this approach works for 1D, 2D, 3D and so on. — it is dimensionally invariant. Since resolution is rarely needed with great accuracy, this gives a convenient method for determining resolution in 2DLC.

The numbers that resulted from multiplying individual column peak capacities showed the tremendous promise for this style of separation. It also fueled speculation in bringing column chromatography methods to bear on the multidimensional system instead of planar separation systems and was a large driving force in the construction of comprehensive 2DLC instrumentation that fully utilized column chromatography (Bushey and Jorgenson, 1990). As discussed below, if the two media used for separation in 2DLC are correlated with respect to the retention mechanism, the peak capacity will be lower than expected from the product approximation.

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