Practical Flow Cytometry by Howard M. Shapiro

By Howard M. Shapiro

From the studies of the third Edition...

"The common reference for an individual attracted to realizing stream cytometry technology."
American magazine of scientific Oncology

"...one of the main useful of its style and...addressed to a large audience?written in such an enticing approach, being either informative and stimulating."
Trends in mobilephone Biology

This reference explains the technological know-how and discusses the mammoth biomedical purposes of quantitative analytical cytology utilizing laser-activated detection and mobilephone sorting. Now in its fourth variation, this article has been increased to supply complete assurance of the huge spectrum of purposes in molecular biology and biotechnology this day. New to this variation are chapters on automatic research of array applied sciences, reimbursement, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, in addition to totally up-to-date and revised references and an inventory of suppliers.Content:

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Sample text

I still had to leave out a lot of good stuff. This edition omits the details of how to build flow cytometers; thousands of people have read the previous editions, in which this material did appear, but only a few dozen people have built “Cytomutts” following the designs in the books. If you’re interested in building an instrument, help is still available; see Chapters 9 and 11. I have put in more, and I hope, better illustrations. They include diagrams, photographs, and displays of flow cytometric data.

I disagreed. The real Bible, or at least the first five books of it, has been dutihlly and faithfully copied by scribes onto parchment scrolls, which are at least as archaic as 8-inch floppy discs, for thousands of years, and continues to present a picture of its time; there are numerous external sources, on paper and in electronic media, to provide translation and commentary, much of it aimed at bringing ancient messages up to date. And, moving from the sublime way over in the direction of the ridiculous, even a book like this one is useful in that it, too, presents a picture of its time; a compilation of the same information on a Web site, frequently updated, will lose historical perspective.

Take, for example, “Flow’s Golden Oldies” as a heading on page 63, or aphorisms like “Shapiro’s First Law of Flow Cytometry: A 5 1 pm Particle CLOGS a 50 pm Orifice”, on page 1 1. I recommend a thorough reading for all who are using and plan to use flow cytometry in analysis and sorting of cells and other biological particles. Leonard A. Herzenberg Stanford University July 29, 1994 PREFACE TO THE THIRD EDITION LARGO A1 FACSTOTUM Each fluorescent label I list in my table, As long as it's stable, dispels the fable; Honestly tells what's in the cells.

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